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Towards Data-driven Fluorescence Microscopy Image Restoration: A Survey
PhD Qualifying Examination Title: "Towards Data-driven Fluorescence Microscopy Image Restoration: A Survey" by Mr. Wenqiang LI Abstract: Fluorescence microscopy is essential in both biological and medical research, offering crucial insights into the structural and functional dynamics of cells, tissues, and complex biological processes. However, issues such as noise, photobleaching, limited resolution, and low imaging speed often hinder image quality and interpretability. Traditional restoration techniques have only partially addressed these issues. Recent advancements in data-driven methods, particularly deep learning, present promising solutions that not only restore but also enhance fluorescence microscopy images beyond conventional limits. This survey reviews state-of-the-art data-driven fluorescence microscopy image restoration, covering fundamental principles, challenges, and advancements in deep learning techniques. We categorize various approaches, highlight key datasets and benchmarking protocols, and examine significant applications in biology and medicine, including pathology and oncology. These techniques have led to breakthroughs by revealing previously undetectable details and improving time resolution. Despite progress, challenges such as the need for diverse datasets, computational costs, and model generalization persist. We discuss emerging trends and future research directions, including real-time image restoration, foundation models, and explainable models. In conclusion, data-driven methods have the potential to transform fluorescence microscopy by pushing the physical limits of this technology. These advancements can significantly enhance image quality and interpretability, thereby expanding the boundaries of scientific discovery and life science exploration. Date: Friday, 16 August 2024 Time: 4:00pm - 6:00pm Zoom Meeting ID: 451 423 0129 Committee Members: Dr. Shuai Wang (Supervisor) Dr. Hao Chen (Chairperson) Dr. Dan Xu Dr. Terence Wong (CBE)